Molecular Control of Circadian Rhythm in Animals


Selected Publications


Zhao, X., Hirota, T., Han, X., Cho, H., Chong, L., Lamia, K., Liu, S., Atkins, A.R., Banayo, E., Liddle, C., Yu, R.T.,
Yates III, J.R., Kay, S.A., Downes, M., and Evans, R.M.: Circadian amplitude regulation via FBXW7-targeted REV-ERBα degradation.
Cell, 165: 1644-1657 (2016)


Lee,J.W.,* Hirota, T.,*,# (*, equal contribution; #, correspondence), Kumar, A.,*,#, Kim, N.J., Irle, S., and Kay, S.A.,#: Development of small molecule Cryptochrome stabilizer derivatives as modulators of the circadian clock. ChemMedChem, 10: 1489-1497 (2015)


Hirota, T. and Kay, S.A.: Identification of small-molecule modulators of the circadian clock.
Methods Enzymol., 551: 267-282 (2015)


St. John, P.C., Hirota, T., Kay, S.A., and Doyle, F.J. 3rd: Spatiotemporal separation of PER and CRY posttranslational regulation in the mammalian circadian clock.
Proc. Natl. Acad. Sci. USA, 111: 2040-2045 (2014)


Hirota, T., Lee, J.W., St. John, P.C., Sawa, M., Iwaisako, K., Noguchi, T., Pongsawakul, P.Y., Sonntag, T., Welsh, D.K., Brenner, D.A., Doyle, F.J. 3rd, Schultz, P.G., and Kay, S.A.: Identification of small molecule activators of cryptochrome.
Science, 337: 1094-1097 (2012)


Lee, J.W.*, Hirota, T.* (*; equal contribution), Peters, E.C., Garcia, M., Gonzalez, R., Cho, C.Y., Wu, X., Schultz, P.G., and Kay, S.A.: A small molecule modulates circadian rhythms through phosphorylation of the period protein.
Angew. Chem. Int. Ed., 50: 10608-10611 (2011)


Hirota, T., Lee, J.W., Lewis, W.G., Zhang, E.E., Breton, G., Liu, X., Garcia, M., Peters, E.C., Etchegaray, J.P., Traver, D., Schultz, P.G., and Kay, S.A.: High-throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIα as a clock regulatory kinase.
PLoS Biol., 8: e1000559 (2010)


Zhang, E.E.*, Liu, Y.*, Dentin, R., Pongsawakul, P.Y., Liu, A.C., Hirota, T., Nusinow, D.A., Sun, X., Landais, S., Kodama, Y., Brenner, D.A., Montminy, M., and Kay, S.A.: Cryptochrome mediates circadian regulation of cAMP signaling and hepatic gluconeogenesis.
Nat. Med., 16: 1152-1156 (2010)


Zhang, E.E.*, Liu, A.C.*, Hirota, T.* (* equal contribution), Miraglia, L.J., Welch, G., Pongsawakul, P.Y., Liu, X., Atwood, A., Huss, J.W. 3rd, Janes, J., Su, A.I., Hogenesch, J.B., and Kay, S.A.: A genome-wide RNAi screen for modifiers of the circadian clock in human cells.
Cell, 139: 199-210 (2009)


Hirota, T. and Kay, S.A.: High-throughput screening and chemical biology: new approaches for understanding circadian clock mechanisms.
Chem. Biol., 16: 921-927 (2009)


Hirota, T., Lewis, W.G., Liu, A.C., Lee, J.W., Schultz, P.G., and Kay, S.A.: A chemical biology approach reveals period shortening of the mammalian circadian clock by specific inhibition of GSK-3b.
Proc. Natl. Acad. Sci. USA, 105: 20746-20751 (2008)