2024.3.20
new
A new study, supported in part by the Live Imaging Center, has been published.
Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei
Plant J (2024) DOI:10.1111/tpj.16699
Akira Yoshinari, Reika Isoda, Noriyoshi Yagi, Yoshikatsu Sato, Jelmer J. Lindeboom, David W. Ehrhardt, Wolf B. Frommer and Masayoshi Nakamura

2024.2.22
new
A new study, supported in part by the Live Imaging Center, has been published.
Plasma membrane damage limits replicative lifespan in yeast and induces premature senescence in human fibroblasts
Nat Aging (2024) 4 319-335 DOI:10.1038/s43587-024-00575-6
Kojiro Suda, Yohsuke Moriyama, Nurhanani Razali, Yatzu Chiu, Yumiko Masukagami, Koutarou Nishimura, Hunter Barbee, Hiroshi Takase, Shinju Sugiyama, Yuta Yamazaki, Yoshikatsu Sato, Tetsuya Higashiyama, Yoshikazu Johmura, Makoto Nakanishi and Keiko Kono

2023.10.22
new
A new study, supported in part by the Live Imaging Center, has been published.
An ABCB transporter regulates anisotropic cell expansion via cuticle deposition in the moss Physcomitrium patens
New Phytologist (2023) 241 665-675 DOI:10.1111/nph.19337
Liechi Zhang, Yuko Sasaki-Sekimoto, Ken Kosetsu, Tsuyoshi Aoyama, Takashi Murata, Yukiko Kabeya, Yoshikatsu Sato, Shizuka Koshimizu, Mie Shimojima, Hiroyuki Ohta, Mitsuyasu Hasebe and Masaki Ishikawa

2023.6.5
new
A new study, supported in part by the Live Imaging Center, has been published.
Root-knot nematode modulates plant CLE3-CLV1 signaling as a long-distance signal for successful infection
Sci. Adv. 9, eadf4803(2023) DOI:10.1126/sciadv.adf4803
Satoru Nakagami, Michitaka Notaguchi, Tatsuhiko Kondo, Satoru Okamoto, Takanori Ida, Yoshikatsu Sato, Tetsuya Higashiyama, Allen Yi-Lun Tsai, Takashi Ishida and Shinichiro Sawa

2023.4.19
old
ITbM's Designated Associate Professor Yoshikatsu Sato, head of the ITbM Live Imaging Center has received the FY2023 Minister for Education, Culture, Sports, Science and Technology's Award for Science and Technology.


Award for Contribution to Research
Yoshikatsu Sato (Designated Associate Professor)
"Contribution to life science research through advanced imaging technology"


"This is the result of exciting research conducted by users of the Live Imaging Center, I deeply appreciate everyone's contributions." said Sato.

2023.1.10
old
A new study, supported in part by the Live Imaging Center, has been published.
CLE3 and its homologues share overlapping functions in the modulation of lateral root formation through CLV1 and BAM1 in Arabidopsis thaliana
Plant J. (2023) 113, 1176-1191 DOI:10.1111/tpj.16103
Satoru Nakagami, Tsuyoshi Aoyama, Yoshikatsu Sato, Taiki Kajiwara, Takashi Ishida and Shinichiro Sawa

2022.10.27
old
A new study, supported in part by the Live Imaging Center, has been published.
Prostaglandin E2 synchronizes lunar-regulated beach spawning in grass puffers
Current Biology (2022) 32, 1-9 DOI:10.1016/j.cub.2022.09.062
Junfeng Chen, Yuma Katada, Kousuke Okimura, Taiki Yamaguchi, Ying-Jey Guh, Tomoya Nakayama, Michiyo Maruyama, Yuko Furukawa, Yusuke Nakane, Naoyuki Yamamoto, Yoshikatsu Sato, Hironori Ando, Asako Sugimura, Kazufumi Tabata, Ayato Sato and Takashi Yoshimura

2022.6.25
old
A new study, supported in part by the Live Imaging Center, has been published.
Rapid Selective Proliferation of Mitochondria during Zygote Maturation in the Uniparental Inheritance of Physarum polycephalum
Cytologia (2022) 87(2): 163?168 DOI:10.1508/cytologia.87.163
Naoki Urakawa, Kakishi Uno, Yoshikatsu Sato, Tetsuya Higashiyama and Narie Sasaki

2022.5.9
old
A new study, supported in part by the Live Imaging Center, has been published.
A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites
Nature Communications (2022)13:2533 DOI:10.1038/s41467-022-30153-6
Keiji Kajiwara, Hiroshi Osaki, Steffen Gresies, Keiko Kuwata, Ju Hyun Kim, Tobias Gensch, Yoshikatsu Sato, Frank Glorius, Shigehiro Yamaguchi and Masayasu Taki

2022.1.5
old
A new study, supported in part by the Live Imaging Center, has been published.
Optical Clearing of Plant Tissues for Fluorescence Imaging
J Vis Exp.(2022)179 DOI:10.3791/63428
Daisuke Kurihara, Yoko Mizuta, Shiori Nagahara, Yoshikatsu Sato and Tetsuya Higashiyama

2021.10.11
old
A new study, supported in part by the Live Imaging Center, has been published.
Dynamics of mitochondrial distribution during development and asymmetric division of rice zygotes
Plant Reproduction (2021) DOI:10.1007/s00497-021-00430-3
Hanifah Aini, Yoshikatsu Sato, Kakishi Uno, Tetsuya Higashiyama and Takashi Okamoto

2021.5.11
old
A new study, supported in part by the Live Imaging Center, has been published.
N-aryl pyrido cyanine derivatives are nuclear and organelle DNA markers for two-photon and super-resolution imaging
Nat Commun (2021) 12 2650 DOI:10.1038/s41467-021-23019-w
Kakishi Uno, Nagisa Sugimoto and Yoshikatsu Sato

2021.4.22
old
A new study, supported in part by the Live Imaging Center, has been published.
Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex
Nat Commun (2021) 12 2331 DOI:10.1038/s41467-021-22661-8
Kazuki Motomura, Hidenori Takeuchi, Michitaka Notaguchi, Haruna Tsuchi, Atsushi Takeda, Tetsu Kinoshita, Tetsuya Higashiyama and Daisuke Maruyama

2021.3.16
old
A new study, supported in part by the Live Imaging Center, has been published.
Trade-off between Plasticity and Velocity in Mycelial Growth
mBio 12:e03196-20. DOI:10.1128/mBio.03196-20
Sayumi Fukuda, Riho Yamamoto, Naoki Yanagisawa, Naoki Takaya, Yoshikatsu Sato, Meritxell Riquelme and Norio Takeshita

2020.12.14
old
A new study, supported in part by the Live Imaging Center, has been published.
Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner
J. Cell Biol (2021) 220 (1): e202005026. DOI:10.1083/jcb.202005026
Kamil So?tysik, Yuki Ohsaki, Tsuyako Tatematsu, Jinglei Cheng, Asami Maeda, Shin-ya Morita and Toyoshi Fujimoto

2020.12.6
old
A new study, supported in part by the Live Imaging Center, has been published.
Two atypical ANGUSTIFOLIA without a plant-specific C-terminus regulate gametophore and sporophyte shapes in the moss Physcomitrium (Physcomitrella) patens
Plant J (2021), 105, 1390-1399 DOI:10.1111/tpj.15121
Katsuaki Takechi, Hiroaki Nagase, Tomoyuki Furuya, Koro Hattori, Yoshikatsu Sato, Kensuke Miyajima, Tomofumi Higuchi, Ryuya Matsuda, Susumu Takio, Hirokazu Tsukaya and Hiroyoshi Takano

2020.12.4
old
A new study, supported in part by the Live Imaging Center, has been published.
Fused Thiophene-S,S-dioxide-Based Super-Photostable Fluorescent Marker for Lipid Droplets
ACS Matt Lett (2021) 3, 42-49 DOI:10.1021/acsmaterialslett.0c00451
Masayasu Taki, Keiji Kajiwara, Eriko Yamaguchi, Yoshikatsu Sato and Shigehiro Yamaguchi

2020.11.20
old
A new study, supported in part by the Live Imaging Center, has been published.
Subnuclear gene positioning through lamina association affects copper tolerance
Nat Commun (2020) 115914 DOI:10.1038/s41467-020-19621-z
Yuki Sakamoto, Mayuko Sato, Yoshikatsu Sato, Akihito Harada, Takamasa Suzuki, Chieko Goto, Kentaro Tamura, Kiminori Toyooka, Hiroshi Kimura, Yasuyuki Ohkawa, Ikuko Hara-Nishimura, Shingo Takagi and Sachihiro Matsunaga

2020.11.12
old
A new study, supported in part by the Live Imaging Center, has been published.
Ran-GTP Is Non-essential to Activate NuMA for Mitotic Spindle-Pole Focusing but Dynamically Polarizes HURP Near Chromosomes
Cur Biol (2021) 31, 115-127.e3 DOI:10.1016/j.cub.2020.09.091
Kenta Tsuchiya, Hisato Hayashi, Momoko Nishina, Masako Okumura, Yoshikatsu Sato, Masato T. Kanemaki, Gohta Goshima and Tomomi Kiyomitsu

2020.10.2
old
A new study, supported in part by the Live Imaging Center, has been published.
Arabidopsis Gex1 is a nuclear membrane protein of gametes required for nuclear fusion during reproduction
Frontiers in Plant Science 11:548032 (2020) DOI:10.3389/fpls.2020.548032
Shuh-ichi Nishikawa, Yuki Yamaguchi, Chiharu Suzuki, Ayaka Yabe, Yuzuru Sato, Daisuke Kurihara, Yoshikatsu Sato, Daichi Susaki, Tetsuya Higashiyama and Daisuke Maruyama

2020.9.27
old
A new study, supported in part by the Live Imaging Center, has been published.
Post‐Assembly Photomasking of Potassium Acyltrifluoroborates (KATs) for Two‐Photon 3D Patterning of PEG‐Hydrogels
Helvetia Chimica Acta 2020, 103, e2000172 DOI:10.1002/hlca.202000172
Haewon Song, Dino Wu, Dimitry Mazunin, Sizhou M. Liu, Yoshikatsu Sato, Nicolas Broguiere, Marcy Zenobi‐Wong and Jeffrey W. Bode

2020.8.14
old
A new study, supported in part by the Live Imaging Center, has been published.
The manifold actions of signaling peptides on subcellular dynamics of a receptor specify stomatal cell fate
eLife, 2020;9:e58097 DOI:10.7554/eLife.58097
Xingyun Qi, Akira Yoshinari, Pengfei Bai, Michal Maes, Scott M Zeng, Keiko U Torii

2020.8.7
old
A new study, supported in part by the Live Imaging Center, has been published.
Cell-cell adhesion in plant grafting is facilitated by β-1,4-glucanases
Science, 369, 698-702 (2020) DOI:10.1126/science.abc3710
Michitaka Notaguchi, Ken-ichi Kurotani, Yoshikatsu Sato, Ryo Tabata, Yaichi Kawakatsu, Koji Okayasu, Yu Sawai, Ryo Okada, Masashi Asahina, Yasunori Ichihashi, Ken Shirasu, Takamasa Suzuki, Masaki Niwa and Tetsuya Higashiyama

2020.8.5
old
A new study, supported in part by the Live Imaging Center, has been published.
Covalent self-labeling of tagged proteins with chemical fluorescent dyes in BY-2 cells and Arabidopsis seedlings
The Plant Cell, 32:3081-3094 (2020) DOI:10.1105/tpc.20.00439
Ryu J. Iwatate, Akira Yoshinari, Noriyoshi Yagi, Marek Grzybowski, Hiroaki Ogasawara, Mako Kamiya, Toru Komatsu, Masayasu Taki, Shigehiro Yamaguchi, Wolf B. Frommer and Masayoshi Nakamura

2020.5.22
old
A new study, supported in part by the Live Imaging Center, has been published.
Phosphole-Oxide-Based Fluorescent Probe for Super-resolution Stimulated Emission Depletion Live Imaging of the Lysosome Membrane
ACS Materials Lett. 2020, 2, 7, 705-711 DOI:10.1021/acsmaterialslett.0c00147
Chenguang Wang, Masayasu Taki*, Keiji Kajiwara, Junwei Wang and Shigehiro Yamaguchi

2019.11.18
old
A new study, supported in part by the Live Imaging Center, has been published.
Hydrophobicity and CH/π-interaction-driven self-assembly of amphiphilic aromatic hydrocarbons into nano sheets
Chemical Communications, in press.DOI:10.1039/C9CC08070H
Tsuyoshi Nishikawa, Hiroki Narita, Soichiro Ogi, Yoshikatsu Sato and Shigehiro Yamaguchi

2019.11.1
old
A new study, supported in part by the Live Imaging Center, has been published.
Two ANGUSTIFOLIA genes regulate gametophore and sporophyte development in Physcomitrella patens
The Plant Journal, in press.DOI:10.1111/tpj.14592
Yoshikazu Hashida, Katsuaki Takechi, Tomomi Abiru, Noriyuki Yabe, Hiroaki Nagase, Koro Hattori, Susumu Takio, Yoshikatsu Sato, Mitsuyasu Hasebe, Hirokazu Tsukaya and Hiroyoshi Takano

2019.10.28
old
A new study, supported in part by the Live Imaging Center, has been published.
AtNOT1 is a novel regulator of gene expression during pollen development
Plant Cell Physiology, 61(4), 712-721 (2019) DOI:10.1093/pcp/pcz235
Kazuki Motomura, Toshihiro Arae, Haruka Araki-Uramoto, Yuya Suzuki, Hidenori Takeuchi, Takamasa Suzuki, Yasunori Ichihashi, Arisa Shibata, Ken Shirasu, Atsushi Takeda, Tetsuya Higashiyama and Yukako Chiba

2019.10.22
old
A new study, supported in part by the Live Imaging Center, has been published.
The formation of perinucleolar bodies is important for normal leaf development and requires the zinc‐finger DNA‐binding motif in Arabidopsis ASYMMETRIC LEAVES2
The Plant Journal, 101, 1118-1134 (2020) DOI:10.1111/tpj.14579
Lilan Luo, Sayuri Ando, Yuki Sakamoto, Takanori Suzuki, Hiro Takahashi, Nanako Ishibashi, Shoko Kojima, Daisuke Kurihara, Tetsuya Higashiyama, Kotaro T. Yamamoto, Sachihiro Matsunaga, Chiyoko Machida, Michiko Sasabe and Yasunori Machida

2019.9.20
old
A new study, supported in part by the Live Imaging Center, has been published.
Moss Kinesin-14 KCBP Accelerates Chromatid Motility in Anaphase
Cell Structure and Function 44, 95-104 DOI:10.1247/csf.19015
Mari W. Yoshida, Moe Yamada and Gohta Goshima

2019.7.23
old
A new study, supported in part by the Live Imaging Center, has been published.
A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae
PNAS, 116 (32) 15817-15822 (2019) DOI:10.1073/pnas.1905924116
Chenguang Wang, Masayasu Taki, Yoshikatsu Sato, Yasushi Tamura, Hideyuki Yaginuma, Yasushi Okada and Shigehiro Yamaguchi

2019.7.19
old
A new study, supported in part by the Live Imaging Center, has been published.
The Effect of Branching on One‐ and Two‐Photon Absorption, Cell Viability and Localization of Cationic Triarylborane Chromophores with Dipolar vs. Octupolar Charge Distributions for Cellular Imaging
Chemistry A European Journal,25, 13164-13175 (2019) DOI:10.1002/chem.201902461
Stefanie Griesbeck, Evripidis Michail, Florian Rauch, Hiraoki Ogasawara, Chenguang Wang, Yoshikatsu Sato, Robert Edkins, Zuolun Zhang, Masayasu Taki, Christoph Lambert, Shigehiro Yamaguchi and Todd B. Marder

2019.7.16
old
A new study, supported in part by the Live Imaging Center, has been published.
Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish
Dev. Biol., 455, 393-408 (2019) DOI:10.1016/j.ydbio.2019.07.010
Takayuki Nimura, Tubasa Itoh, Hanako Hagio, Takuto Hayashi, Vincenzo Di Donato, Miki Takeuchi, Takeaki Itoh, Fuduki Inoguchi, Yoshikatsu Sato, Naoyuki Yamamoto, Yu Katsuyama, Filippo Del Bene, Takashi Shimizu and Masahiko Hibi

2019.7.10
old
A new study, supported in part by the Live Imaging Center, has been published.
Microtubule depletion domain 1 localizes at the boundary between female gametes in Arabidopsis thaliana
Mol. Reprod. Dev., 86, 8, 925-925 (2019) DOI:10.1002/mrd.23175
Hiroki Tsutsui, Yoshikatsu Sato, Daichi Susaki and Tetsuya Higashiyama

2019.7.8
old
A new study, supported in part by the Live Imaging Center, has been published.
Physcomitrella STEMIN transcription factor induces stem cell formation with epigenetic reprogramming
Nature Plants 5, 681-690 DOI:10.1038/s41477-019-0464-2
Masaki Ishikawa, Mio Morishita, Yohei Higuchi, Shunsuke Ichikawa, Takaaki Ishikawa, Tomoaki Nishiyama, Yukiko Kabeya, Yuji Hiwatashi, Tetsuya Kurata, Minoru Kubo, Shuji Shigenobu, Yosuke Tamada, Yoshikatsu Sato and Mitsuyasu Hasebe

2019.5.5
old
A new study, supported in part by the Live Imaging Center, has been published.
Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the moss Physcomitrella patens
eLIFE 8, e43652 DOI:10.7554/eLife.43652
Elena Kozgunova, Momoko Nishina and Gohta Goshima

2019.4.30
old
A new study, supported in part by the Live Imaging Center, has been published.
Characterization of the Nicotianamine Exporter ENA1 in Rice
Frontiers in Plant Science,10 (2019) DOI:10.3389/fpls.2019.00502
Tomoko Nozoye, Nicolaus von Wiren, Yoshikatsu Sato, Tetsuya Higashiyama, Hiromi Nakanishi and Naoko K. Nishizawa

2019.4.23
old
A new study, supported in part by the Live Imaging Center, has been published.
Tuning the π-bridge of quadrupolar triarylborane chromophores for one- and two-photon excited fluorescence imaging of lysosomes in live cells
Chemical Science,10, 5405-5422 (2019) DOI:10.1039/C9SC00793H
Stefanie Griesbeck, Michail Evripidis, Chenguang Wang, Hiroaki Ogasawara, Sabine Lorenzen, Lukas Gerstner, Theresa Zang, Jorn Nitsch, Yoshikatsu Sato, Rudiger Bertermann, Masayasu Taki, Christoph Lambert, Shigehiro Yamaguchi and Todd B. Marder

2019.2.5
old
A new study, supported in part by the Live Imaging Center, has been published.
Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes
Proc Natl Acad Sci USA, 116, 2338-2343 (2019) DOI:10.1073/pnas.1814160116
Yusuke Kimata, Takehide Kato, Takumi Higaki, Daisuke Kurihara, Tomomi Yamada, Shoji Segami, Miyo Terao Morita, Masayoshi Maeshima, Seiichiro Hasezawa, Tetsuya Higashiyama, Masao Tasaka and Minako Ueda

2019.1.28
old
A new study, supported in part by the Live Imaging Center, has been published.
Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis.
Nat Commun. 10, 473 (2019) DOI:10.1038/s41467-019-08411-x
Kamil Soltysik, Yuki Ohsaki, Tsuyako Tatematsu, Jinglei Cheng and Toyoshi Fujimoto

2018.11.28
old
A new study, supported in part by the Live Imaging Center, has been published.
The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants
Development, 145, 170076 (2018) DOI:10.1242/dev.170076
Taro Takahashi, Toshiyuki Mori, Kenji Ueda, Lixy Yamada, Shiori Nagahara, Tetsuya Higashiyama, Hitoshi Sawada and Tomoko Igawa

2018.9.26
old
A new study, supported in part by the Live Imaging Center, has been published.
UBL3 modification influences protein sorting to small extracellular vesicles
Nature Communications 9: 3936 (2018) DOI:10.1038/s41467-018-06197-y
Hiroshi Ageta, Natsumi Ageta-Ishihara, Keisuke Hitachi, Ozge Karayel, Takanori Onouchi, Hisateru Yamaguchi, Tomoaki Kahyo, Ken Hatanaka, Koji Ikegami, Yusuke Yoshioka, Kenji Nakamura, Nobuyoshi Kosaka, Masashi Nakatani, Akiyoshi Uezumi, Tomihiko Ide, Yutaka Tsutsumi, Haruhiko Sugimura, Makoto Kinoshita, Takahiro Ochiya, Matthias Mann, Mitsutoshi Setou and Kunihiro Tsuchida

2018.8.6
old
A new study, supported in part by the Live Imaging Center, has been published.
A Highly Photostable Near‐Infrared Labeling Agent Based on a Phospha‐rhodamine for Long‐Term and Deep Imaging
Angew. Chem. Int. Ed. 57(32):10137-10141 (2018) DOI:10.1002/anie.201804731
Marek Grzybowski, Masayasu Taki, Kieko Senda, Yoshikatsu Sato, Tetsuro Ariyoshi, Yasushi Okada, Ryosuke Kawakami, Takeshi Imamura and Shigehiro Yamaguchi

2018.5.23
old
A new study, supported in part by the Live Imaging Center, has been published.
Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces
J. Vis. Exp. (135), e57262 (2018) DOI:10.3791/57262
Naoki Yanagisawa, Nagisa Sugimoto, Tetsuya Higashiyama and Yoshikatsu Sato

2018.5.17
old
A new study, supported in part by the Live Imaging Center, has been published.
Repetitive stretching of giant liposomes utilizing the nematic alignment of confined actin
Communications Physics 1; 18 (2018) DOI:10.1038/s42005-018-0019-2
Shunsuke Tanaka, Kingo Takiguchi and Masahito Hayashi

2018.4.17
old
A new study, supported in part by the Live Imaging Center, has been published.
Peanut agglutinin specifically binds to a sperm region between the nucleus and mitochondria in tunicates and sea urchins
Molecular Reproduction and Development, 85, 6, 464-477 (2018) DOI:10.1002/mrd.22982
Shiori Nakazawa, Maki Shirae‐Kurabayashi and Hitoshi Sawada

2018.3.30
old
A new study, supported in part by the Live Imaging Center, has been published.
Quantitative assessment of chemotropism in pollen tubes using microslit channel filters
Biomicrofluidics 12: 24113 (2018) DOI:10.1063/1.5023718
Naoki Yanagisawa and Tetsuya Higashiyama

2018.2.26
old
A new study, supported in part by the Live Imaging Center, has been published.
Direct and Indirect Interlayer Excitons in a van der Waals Heterostructure of hBN/WS2/MoS2/hBN
ACS Nano 2018, 12, 2498-2505 DOI:10.1021/acsnano.7b08253
Mitsuhiro Okada, Alex Kutana, Yusuke Kureishi, Yu Kobayashi, Yuika Saito, Tetsuki Saito, Kenji Watanabe, Takashi Taniguchi, Sunny Gupta, Yasumitsu Miyata, Boris I. Yakobson, Hisanori Shinohara and Ryo Kitaura

2018.2.1
old
A new study, supported in part by the Live Imaging Center, has been published.
Capacitation in Plant and Animal Fertilization
Trends in Plant Science 23: 129-139 (2018) DOI:10.1016/j.tplants.2017.10.006
Subramanian Sankaranarayanan and Tetsuya Higashiyama

2018.2.1
old
A new study, supported in part by the Live Imaging Center, has been published.
Pollen differentiation as well as pollen tube guidance and discharge are independent of the presence of gametes
Development 145: 152645 (2018) DOI:10.1242/dev.152645
Barbara Glockle, Wojciech J. Urban, Shiori Nagahara, Ellen D. Andersen, Tetsuya Higashiyama, Paul E. Grini and Arp Schnittger

2018.1.31
old
A new study, supported in part by the Live Imaging Center, has been published.
A Water-soluble Warped Nanographene: Synthesis and Applications for Photo-induced Cell Death
Angew. Chem. Int. Ed. 2018 Mar 5;57(11):2874-2878 DOI:10.1002/anie.201713387
Hsing-An Lin, Yoshikatsu Sato, Yasutomo Segawa, Taishi Nishihara, Nagisa Sugimoto, Lawrence T. Scott, Tetsuya Higashiyama and Kenichiro Itami

2018.1.29
old
A new study, supported in part by the Live Imaging Center, has been published.
A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm
J Cell Sci., jcs204123 DOI:10.1242/jcs.204123
Kazuki Motomura, Tomokazu Kawashima, Frederic Berger, Tetsu Kinoshita, Tetsuya Higashiyama and Daisuke Maruyama

2018.1.11
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A new study, supported in part by the Live Imaging Center, has been published.
A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging
Chem Commun(Camb). 2018 Jan 2;54(3):299-302 DOI:10.1039/C7CC07344E
Hiroaki Ogasawara, Marek Grzybowski, Riho Hosokawa, Yoshikatsu Sato, Masayasu Taki and Shigehiro Yamaguchi

2018.1.8
old
A new study, supported in part by the Live Imaging Center, has been published.
ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Development. 2018 Jan 8;145, dev156380 DOI:10.1242/dev.156380
Yuka Kimura, Masao Tasaka, Keiko U. Torii and Naoyuki Uchida

2017.9.27
old
Super-photostable fluorescent dye, "C-Naphox-TEG”, developed from Yamaguchi Group in ITbM is now on sale.


Product #: D5204
Cas #: 1802050-34-5


Reference:A Phosphole Oxide Based Fluorescent Dye with Exceptional Resistance to Photobleaching: A Practical Tool for Continuous Imaging in STED Microscopy
Angew. Chem. Int. Ed. 2015, 54, 15213-15217. DOI:10.1002/anie.201507939
C. Wang, A. Fukazawa, M. Taki, Y. Sato, T. Higashiyama and S. Yamaguchi

2017.9.12
old
A new study, supported in part by the Live Imaging Center, has been published.
Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells
Scientific Reports 7 11257 (2017)DOI:10.1038/s41598-017-10843-8
Taeko Sasaki, Yoshikatsu Sato, Tetsuya Higashiyama and Narie Sasaki

2017.9.11
old
A new study, supported in part by the Live Imaging Center, has been published.
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
Journal of Visualized Experiments, 55975 (2017) DOI:10.3791/55975
Daisuke Kurihara, Yusuke Kimata, Tetsuya Higashiyama and Minako Ueda

2017.9.6
old
A new study, supported in part by the Live Imaging Center, has been published.
Fluorescent Labeling of the Cyst Nematode Heterodera glycines in Deep-Tissue Live Imaging
CYTOLOGIA 82(3) 251-259 (2017) DOI:10.1508/cytologia.82.251
Mina Ohtsu, Daisuke Kurihara, Yoshikatsu Sato, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama and Tetsuya Higashiyama

2017.8.5
old
A new study, supported in part by the Live Imaging Center, has been published.
Drosophila Dalmatian combines sororin and shugoshin roles in establishment and protection of cohesion
The EMBO Journal (2017) 36, 1513-1527DOI:10.15252/embj.201695607
Takashi Yamada, Eri Tahara, Mai Kanke, Keiko Kuwata and Tomoko Nishiyama

2017.7.25
old
A new study, supported in part by the Live Imaging Center, has been published.
Super-Photostable Phosphole-Based Dye for Multiple-Acquisition Stimulated Emission Depletion Imaging
Journal of the American Chemical Society (2017) 139(30), 10374-10381 DOI:10.1021/jacs.7b04418
Chenguang Wang, Masayasu Taki, Yoshikatsu Sato, Aiko Fukazawa, Tetsuya Higashiyama and Shigehiro Yamaguchi

2017.5.22
old
A new study, supported in part by the Live Imaging Center, has been published.
Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
Scientific Reports 7, 1909 (2017) DOI:10.1038/s41598-017-01786-1
Yoshikatsu Sato, Nagisa Sugimoto, Tadayoshi Hirai, Akihiro Imai, Minoru Kubo, Yuji Hiwatashi, Tomoaki Nishiyama and Mitsuyasu Hasebe

2017.5.9
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A new study, supported in part by the Live Imaging Center, has been published.
Capability of tip-growing plant cells to penetrate into extremely narrow gaps
Scientific Reports 7, 1403 (2017) DOI:10.1038/s41598-017-01610-w
Naoki Yanagisawa, Nagisa Sugimoto, Hideyuki Arata, Tetsuya Higashiyama and Yoshikatsu Sato

2017.4.21
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A new study, supported in part by the Live Imaging Center, has been published.
Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote
Genes & Development (2017) 31, 617-627 DOI:10.1101/gad.292409.116
Minako Ueda, Ernst Aichinger, Wen Gong, Edwin Groot, Inge Verstraeten, Lam Dai Vu, Ive De Smet, Tetsuya Higashiyama, Masaaki Umeda and Thomas Laux

2017.3.25
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A new study, supported in part by the Live Imaging Center, has been published.
Spatiotemporal deep-imaging of syncytium induced by the soybean cyst nematode Heterodera glycines
Protoplasma, November 2017, Volume 254, Issue 6, pp2107-2115 DOI:10.1007/s00709-017-1105-0
Mina Ohtsu, Yoshikatsu Sato, Daisuke Kurihara, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama and Tetsuya Higashiyama

2017.3.10
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A new study, supported in part by the Live Imaging Center, has been published.
ERECTA-family receptor kinase genes redundantly prevent premature progression of secondary growth in the Arabidopsis hypocotyl
New Phytologist (2017) Vol.213, 1697-1709 DOI:10.1111/nph.14335
Shuka Ikematsu, Masao Tasaka, Keiko U. Torii and Naoyuki Uchida

2017.1.17
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A new study, supported in part by the Live Imaging Center, has been published.
Key Structural Elements of Unsymmetrical Cyanine Dyes for Highly Sensitive Fluorescence Turn-on DNA Probe
Chem. Asian J. (2017) Vol.12, 233-238 DOI:10.1002/asia.201601430
Kakishi Uno, Taeko Sasaki, Nagisa Sugimoto, Hideto Ito, Taishi Nishihara, Shinya Hagihara, Tetsuya Higashiyama, Narie Sasaki, Yoshikatsu Sato and Kenichiro Itami

2016.12.15
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A new study, supported in part by the Live Imaging Center, has been published.
Fertilization-independent cell-fusion between the synergid and central cell in the polycomb mutant
Cell Structure and Function (2016) 41, 121-125 DOI:10.1247/csf.16010
Kazuki Motomura, Frederic Berger, Tomokazu Kawashima, Tetsu Kinoshita, Tetsuya Higashiyama and Daisuke Maruyama

2016.11.24
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A new study, supported in part by the Live Imaging Center, has been published.

Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote
PNAS (2016) 113, 14157-62 DOI:10.1073/pnas.1613979113
Yusuke Kimata, Takumi Higaki, Tomokazu Kawashima, Daisuke Kurihara, Yoshikatsu Sato, Tomomi Yamada, Seiichiro Hasezawa, Frederic Berger, Tetsuya Higashiyama and Minako Ueda

2016.11.4
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A new study, supported in part by the Live Imaging Center, has been published.
Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization
Science Advances 28 Oct 2016: Vol. 2, no. 10, e1600554 DOI:10.1126/sciadv.1600554
Ryushiro D. Kasahara, Michitaka Notaguchi, Shiori Nagahara, Takamasa Suzuki, Daichi Susaki, Yujiro Honma, Daisuke Maruyama and Tetsuya Higashiyama

2016.9.5
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A new study, supported in part by the Live Imaging Center, has been published.
A secreted peptide and its receptors shape the auxin response pattern and leaf margin morphogenesis
Current Biology (2016) 26, 2478-2485 DOI:10.1016/j.cub.2016.07.014
Toshiaki Tameshige, Satoshi Okamoto, Jin Suk Lee, Mitsuhiro Aida, Masao Tasaka, Keiko U. Torii and Naoyuki Uchida

2016.7.14
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A new study, supported in part by the Live Imaging Center, has been published.
Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in S. cerevisiae
PNAS (2016) 113, 6910-5 DOI:10.1073/pnas.1523824113
Keiko Kono, Amr Al-Zain, Lea Schroeder, Makoto Nakanishi and Amy E.Ikui

2016.5.9
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A new study, supported in part by the Live Imaging Center, has been published.
A Macrocyclic Fluorophore Dimer with Flexible Linkers: Bright Excimer Emission with a Long Fluorescence Lifetime
Angew Chem. (2016) 55, 7131-5 DOI:10.1002/anie.201602239
Hiroshi Osaki, Chih-Ming Chou, Masayasu Taki, Kai Welke, Daisuke Yokogawa, Stephan Irle, Yoshikatsu Sato, Tetsuya Higashiyama, Shohei Saito, Aiko Fukazawa and Shigehiro Yamaguchi

2016.4.11
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A new study, supported in part by the Live Imaging Center, has been published.
The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance
Current Biology (2016) 26, 1091-7 DOI:10.1016/j.cub.2016.02.040
Akane G. Mizukami, Rie Inatsugi, Jiao Jiao, Toshihisa Kotake, Keiko Kuwata, Kento Ootani, Satohiro Okuda,Subramanian Sankaranarayanan, Yoshikatsu Sato, Daisuke Maruyama, Hiroaki Iwai, Estelle Garenaux, Chihiro Sato, Ken Kitajima,Yoichi Tsumuraya, Hitoshi Mori, Junichiro Yamaguchi, Kenichiro Itami, Narie Sasaki and Tetsuya Higashiyama

2016.4.7
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A new study, supported in part by the Live Imaging Center, has been published.
Intra-spindle microtubule assembly regulates clustering of microtubule-organizing centers during early mouse development
Cell Reports (2016) 15, 54-60 DOI:10.1016/j.celrep.2016.02.087
Sadanori Watanabe, Go Shioi, Yasuhide Furuta and Gohta Goshima

 
2016.3.28
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Yoshimulacton Green is now on sale.


Product #: E1238
Cas #: 1807549-00-3


Reference:Probing strigolactone receptors in Striga hermonthica with fluorescence
Science 2015, 349, 864. DOI:10.1126/science.aab3831
Yuichiro Tsuchiya, Masahiko Yoshimura, Yoshikatsu Sato, Keiko Kuwata, Shigeo Toh, Duncan Holbrook-Smith, Hua Zhang, Peter McCourt, Kenichiro Itami, Toshinori Kinoshita and Shinya Hagihara

2016.3.10
old
A new study, supported in part by the Live Imaging Center, has been published.
Tip-localized receptors control pollen tube growth and LURE sensing in Arabidopsis
Nature, 531, 245-248, (2016). DOI:10.1038/nature17413
Hidenori Takeuchi and Tetsuya Higashiyama

2016.2.25
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A new study, supported in part by the Live Imaging Center, has been published.
Haspin kinase has multiple functions in the plant cell division regulatory network
Plant Cell Physiology (2016) 57, 848-861 DOI:10.1093/pcp/pcw030
Elena Kozgunova, Takamasa Suzuki, Masaki Ito, Tetsuya Higashiyama and Daisuke Kurihara

                
2016.1.21
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A new study, supported in part by the Live Imaging Center, has been published.


Phospha-fluorescein: A red-emissive fluorescein analogue with high photobleaching resistance
Chem. Commun.(2016) 52, 1120-1123 DOI:10.1039/C5CC09345G
Aiko Fukazawa, Shinji Suda, Masayasu Taki, Eriko Yamaguchi, Marek Grzybowski, Yoshikatsu Sato, Tetsuya Higashiyama and Shigehiro Yamaguchi

2015.12.8
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A new study, supported in part by the Live Imaging Center, has been published.


ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging
Development (2015) 142, 4168-4179 DOI: 10.1242/dev.127613
Daisuke Kurihara, Yoko Mizuta, Yoshikatsu Sato and Tetsuya Higashiyama

                
2015.12.3
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A new study, supported in part by the Live Imaging Center, has been published.
Lactic acid is a sperm motility inactivation factor in the sperm storage tubules
Scientific Reports (2015) 5, 17643. DOI:10.1038/srep17643
Mei Matsuzaki, Shusei Mizushima, Gen Hiyama, Noritaka Hirohash, Kogiku Shiba, Kazuo Inaba, Tomohiro Suzuki, Hideo Dohra, Toshiyuki Ohnishi, Yoshikatsu Sato, Tetsuya Kohsaka, Yoshinobu Ichikawa and Tomohiro Sasanami

2015.12.3
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A new study, supported in part by the Live Imaging Center, has been published.
Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway
J. Biosci. Bioeng. (2015) 120(6):697-700. DOI:10.1016/j.jbiosc.2015.03.021
Yoshikatsu Sato, Nagisa Sugimoto, Tetsuya Higashiyama and Hideyuki Arata

                
2015.10.26
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A new study, supported in part by the Live Imaging Center, has been published.
A Phosphole Oxide Based Fluorescent Dye with Exceptional Resistance to Photobleaching: A Practical Tool for Continuous Imaging in STED Microscopy
Angew Chem. (2015) DOI: 10.1002/anie.201507939
Chenguang Wang, Aiko Fukazawa, Masayasu Taki, Yoshikatsu Sato, Tetsuya Higashiyama and Shigehiro Yamaguchi

2015.10.22
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A new study, supported in part by the Live Imaging Center, has been published.
Type IV Collagen Controls the Axogenesis of Cerebellar Granule Cells by Regulating Basement Membrane Integrity in Zebrafish
PLOS GENETICS (2015) 11(10), E1005587. DOI: 10.1371/journal.pgen.1005587
Miki Takeuchi, Shingo Yamaguchi, Shigenobu Yonemura, Kisa Kakiguchi, Yoshikatsu Sato, Tetsuya Higashiyama, Takashi Shimizu and Masahiko Hibi

2015.10.21
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A new study, supported in part by the Live Imaging Center, has been published.
Live Imaging and Laser Disruption Reveal the Dynamics and Cell-Cell Communication During Torenia fournieri Female Gametophyte Development
Plant Cell Physiol. (2015) 56, 1031-1041. DOI: 10.1093/pcp/pcv031
Daichi Susaki, Hidenori Takeuchi, Hiroki Tsutsui, Daisuke Kurihara and Tetsuya Higashiyama

2015.8.21
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A new study, supported in part by the Live Imaging Center, has been published.
Probing strigolactone receptors in Striga hermonthica with fluorescence
Science (2015) 349, 6520, 864-868. DOI: 10.1126/science.aab3831
Yuichiro Tsuchiya, Masahiko Yoshimura, Yoshikatsu Sato, Keiko Kuwata, Shigeo Toh, Duncan Holbrook-Smith, Hua Zhang, Peter McCourt, Kenichiro Itami, Toshinori Kinoshita and Shinya Hagihara

2015.7.9
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A new study, supported in part by the Live Imaging Center, has been published.
Live-Cell Imaging and Optical Manipulation of Arabidopsis Early Embryogenesis
Developmental Cell (2015) DOI: 10.1016/j.devcel.2015.06.008
Keita Gooh, Minako Ueda, Kana Aruga, Jongho Park, Hideyuki Arata, Tetsuya Higashiyama and Daisuke Kurihara

2015.7.2
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A new study, supported in part by the Live Imaging Center, has been published.
A red-emitting ratiometric fluorescent probe based on a benzophosphole P-oxide scaffold for the detection of intracellular sodium ion
Chem. Commun. (2015) DOI: 10.1039/C5CC03547C
Masayasu Taki, Hiroaki Ogasawara, Hiroshi Osaki, Aiko Fukazawa, Yoshikatsu Sato, Kimi Ogasawara, Tetsuya Higashiyama and Shigehiro Yamaguchi

2015.4.24
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A new study, supported in part by the Live Imaging Center, has been published.
Rapid elimination of the persistent synergid through a cell fusion mechanism
Cell (2015) DOI: 10.1016/j.cell.2015.03.018
Daisuke Maruyama, Ronny Volz, Hidenori Takeuchi, Toshiyuki Mori, Tomoko Igawa, Daisuke Kurihara, Tomokazu Kawashima, Minako Ueda, Masaki Itoh, Masaaki Umeda, Shuh-ichi Nishikawa, Rita Gros-Hardt and Tetsuya Higashiyama

2015.3.17
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A new study, supported in part by the Live Imaging Center, has been published.


Environment-Sensitive Fluorescent Probe: A Benzophosphole Oxide with an Electron-Donating Substituent
Angew Chem Int Ed Engl., 7;54(15):4539-43 (2015).
Eriko Yamaguchi, Chenguang Wang, Aiko Fukazawa, Masayasu Taki, Yoshikatsu Sato,Taeko Sasaki, Minako Ueda, Narie Sasaki, Tetsuya Higashiyama and Shigehiro Yamaguchi

2015.3.4
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A new study, supported in part by the Live Imaging Center, has been published.
Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene
Plant Reproduction, 2015; 28(1): 33?46.
Shiori Nagahara, Hidenori Takeuchi and Tetsuya Higashiyama
2014.12.16
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A new study, supported in part by the Live Imaging Center, has been published.
The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site
Journal of Plant Research, 128 327-336 (2015)
Michiko Sasabe, Nanako Ishibashi, Tsuyoshi Haruta, Aki Minami, Daisuke Kurihara, Tetsuya Higashiyama, Ryuichi Nishihama, Masaki Ito and Yasunori Machida
2014.9.2
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A new study, supported in part by the Live Imaging Center, has been published.
Live imaging of calcium spikes during double fertilization in Arabidopsis
Nature Commun. 5: 4722 (2014).
Yuki Hamamura, Moe Nishimaki, Hidenori Takeuchi, Anja Geitmann, Daisuke Kurihara and Tetsuya Higashiyama
2014.6.16
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A new study, supported in part by the Live Imaging Center, has been published.
Kinesins have a dual function in organizing microtubules during both tip growth and cytokinesis in Physcomitrella patens
Plant Cell 26: 1256-1266 (2014).
Yuji Hiwatashi, Yoshikatsu Sato and John H. Doonan
2014.3.11
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A new study, supported in part by the Live Imaging Center, has been published.
Spatial distribution of the RABBIT EARS protein and effects of its ectopic expression in Arabidopsis thaliana flowers
Planta Volume 239 Issue 3 (2014) Page 707-715
Seiji Takeda, Mariko Noguchi, Yuki Hamamura and Tetsuya Higashiyama
2014.2.28
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Live imaging center website has been updated.



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