Mako Kamiya, PhD
Professor, Department of Life Science and Technology, Tokyo Institute of Technology
Lecture Title
Molecular design of activatable Raman probes for multiplexed bioimaging
Abstract
Raman probes based on alkyne or nitrile tags hold promise for highly multiplexed imaging. In order to functionalize the Raman probes to be responsive to biological phenomena in live cells, we have established a general strategy to control Raman signals intensity based on the resonant Raman effect that show enhanced stimulated Raman scattering (SRS) signals upon reaction with enzymes under physiological conditions. By optimizing xanthene derivative bearing a nitrile group at 9th position, we synthesized four activatable Raman probes for different enzymes with different vibrational frequencies and succeeded in simultaneous imaging of these enzyme activities in live cells.[1] We recently expanded our molecular design strategy by combining the resonant Raman effect and aggregate formation to develop new series of activatable Raman probes. We focused on a rhodol derivative bearing a nitrile group at 9th position, 9CN-JCR, which shows increased SRS signal intensity and high aggregate-forming ability after probe activation, resulting in good retention in target cells. By using isotope-edited 9CN-JCR-based probes, we could simultaneously detect three enzyme activities in live cultured cells, and distinguish cell regions expressing target enzyme activity in live Drosophila wing disc and fat body ex vivo.[2] Further, based on the established molecular design strategy, we succeeded to develop photoactivatable probes[3] and photoswitchable Raman probes for super-resolution vibrational imaging.[4] In this symposium, I would like to introduce our molecular design strategy and their application to live cells and tissues.
References:
- Fujioka H, Shou J, Kojima R, Urano Y, Ozeki Y*, Kamiya M*: J. Am. Chem. Soc. 142, 20701-20707 (2020).
- Fujioka H, Kawatani M, Spratt S, Komazawa A, Misawa Y, Shou J, Mizuguchi T, Kosakamoto H, Kojima R, Urano Y, Obata F, Ozeki Y, Kamiya M*: J. Am. Chem. Soc. 145, 8871–8881 (2023).
- Kawatani M, Spratt SJ, Fujioka H, Shou J, Misawa Y, Kojima R, Urano Y, Ozeki Y, Kamiya M*: Chem. Asian J. 18, e202201086 (2023).
- Shou J, Komazawa A, Wachi Y, Kawatani M, Fujioka H, Spratt SJ, Mizuguchi T, Oguchi K, Akaboshi H, Obata F, Tachibana R, Yasunaga S, Mita Y, Misawa Y, Kojima R, Urano Y, Kamiya M*, Ozeki Y*: Sci. Adv. 9, eade9118 (2023).