GTR Seminar / Topics in Advanced Biological Science
Date:May 29(Wed),2024,17:00-18:30
Venue:Lecture room 131,Science E Building
Speaker:Dr. Ananthanarayan Kumar (Researcher, The Discovery Centre, AstraZeneca, Cambridge, UK)
Title:Utilizing an Integrative Structural Biology Strategy to Visualize Complex Nucleic Acid Machinery
Langage: English
Host:Seiji Kojima kojima.seiji.z6<at>f.mail.nagoya-u.ac.jp(<at>→@）
Abstract:The central dogma of molecular biology dictates that genetic information is transcribed into messenger RNA (mRNA), which serves as a template for protein synthesis. However, less than 2% of the human transcriptome comprises mRNAs; the majority consists of non-coding RNAs devoid of protein-coding capability. In the first part, I will talk about the mechanistic intricacies behind mRNA polyadenylation catalyzed by the cleavage and polyadenylation factor (CPF), revealed by biochemical reconstitution, electron cryo-microscopy, biophysical assays, and NMR spectroscopy1). In the second part, I will describe the architecture of the human long non-coding RNA NORAD (Non-Coding RNA Activated By DNA Damage). Employing an integrative structural biology approach merging chemical probing, RNA-pull downs coupled with mass spectrometry, I unveiled a structured protein interaction hub within NORAD's functional core2).
References 1) Kumar et al, Genes Dev., 35:1510-1226, 2021: 2) BioRxiv.
submitted.
★GTR international students only
This seminar is "GTR Lecture series on multidisciplinary problems (1pt)".
Enroll in gtr e-portfolio (Dead line: May.26)．Grades will be judged by report. Report submission from TACT by June 12.