GTR Seminar
１月１９日（木）に下記の通りGTRセミナーを開催いたします。
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LIU教授は植物の硝酸イオンシグナル伝達機構の解明において世界をリードする研究を展開しています。
日時：2023年1月19日（木） 15:00 - 16:30
会場：理農館SA329
言語：英語
講演者：Kun-Hsiang LIU 教授 Northwest Agriculture and Forestry University/MGH, USA
講演タイトル：Exploring novel regulators in nitrate signaling
Most higher plants prefer to utilize nitrate as their primary form of nitrogen source. Besides being a macronutrient, nitrate also serves as a signal molecule. Nitrate induces nitrate responsive gene expression and promotes root elongation and leaf development. However, it is still not clear how plants sense nitrate and what is the molecular mechanisms of nitrate signaling. In our previous study, we found that nitrate triggers unique calcium waves and that calcium-dependent protein kinases (CPKs) act as calcium sensors to transduce nitrate signals. Furthermore, the key transcription factor NLP7 can be directly phosphorylated by
CPK10/30/32 and retained in the nucleus to activate primary nitrate-responsive gene expression. Recently, we demonstrated that NLP transcription factors are the master regulators in nitrate signaling.
From NLP-NIN chimera analysis, the N-terminus of NLP7 acts as a repressive domain in full-length NLP7 and is derepressed by nitrate.
Additionally, we further show that NLP7 can bind to nitrate and act as a nitrate sensor. To visualize nitrate binding, we generated a genetically encoded fluorescent biosensor (sCiNiS) to detect nitrate binding in plants. The nitrate sensor domain of NLP7 is similar to the bacterial nitrate sensor NreA. Substitution of conserved residues in the ligand-binding pocket impairs the nitrate-triggered ability of NLP7 function.
Liu et al. Nature 545: 311-316 (2017)
Liu et al. Science 377: 1419-1425 (2022)
問合せ先：榊原均（sakaki<at>agr.nagoya-u.ac.jp(<at>→@に変換)）
共催：研究⼤学強化促進事業・最先端国際研究ユニット「最先端情報分⼦・植物最適⾏動統御ユニット」
★このセミナーは外国人GTR学生向けGTRシリーズ講義です。レポートを提出し合格した外国人GTR生には、シリーズ講義1ポイントが
付与されます。レポートはNUCTから提出いただきます。受講を希望する場合はGTR e-ポートフォリオにも登録してください。
登録締め切り：11月16日